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KMID : 0355319920150010033
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Journal of Korean Academy of Oral Pathology
1992 Volume.15 No. 1 p.33 ~ p.48
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THE EFFECT OF GANODERMA LUCIDUM AND AROMATIC RETINOID ON CARCINOGENESIS IN GINGIVA OF RAT
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유석규/Seok Kyu Yoo
조한국/Han Kuk Cho
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Abstract
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-Abstract-
The purpose of this study is to evaluate the tumor inhibitory effects of Ganoderma
lucidum and aromatic retinoid no the carcinogenesis induced by implantation of 9,
10-dimethyl-1,2-benzanthracene(DMBA, Sigma chemical Co.) into the mandibular gingiva
of rats.
One hundred and twenty six Sprague-Dawley rats. 3 months of age and 100-120gm in
weight were used for this experiment, and these rats were devided into 3 experimental
groups and 2 control groups. The gingiva of the right mandible of 90 rats were incised
and 4 mg pellet of DMBA were implanted into incised gingivae(Group 1, 2, 3).
30 rats were supplied with 1.0ml Ganderma lucidum extract administered orally by a
stomach tube for 3 times per week(Group 2). 30 rats were administered Ganoderma
lucidum extract by the same method of Group 2 and combined with aromatic
retinoid(Ro10-9359) at 6, 10, 12, 16, 18 and 20 weeks after experiment
respectively. When sacrificed, the right mandible were removed and fixed in 10％
N-formalin. After decalcification with 5％ nitric acid, embedded in paraffin, sectioned to
5㎛ thickness and stained with Hematoxylin-Eosin stain and immunohistochemistry for
tumor necrosis factor alpha(TNF-α).
The obtained results were as follows ;
1. DMBA-implanted group(Group 1) revealed epithelial dysplasia at 8weeks, infiltrative
proliferation into underlying connective tissue at 10 weeks, well differentiated squamous
cell carcinoma at 12 weeks and developed poorly differentiated squamous cell carcinoma
at 16 to 20 weeks.
2. Group 2 revealed slight epithelial dysplasia at 8 weeks, carcinoma in situ at 12
weeks, infiltrative squamous cell carcinoma, numerous lymphatic channels and infiltration
of imflammatory cells at 16 weeks and infiltrative squamous cell carcinoma and well
differentiated fibrosarcoma from 18 weeks. Development of squamous cell carcinoma
delayed 2-4 weeks than that group 1.
3. Group 3 revealed epithelial dysplasia at 8 weeks, infiltrated carcinoma in situ at 12
weeks. Tumor size decreased in gross and histologically desquamation of neoplastic
cellualr region by necrosis of underlying connective tissue were observed from 16
weeks.
4. Fibrosarcoma developed which is combined with squamous cell carcinoma, and
development of these in group 2 and 3 delayed 2-4 weeks than that group 1.
5. In control group, Group 4 revealed inflammatory reaction and atrophy of epithelium.
6. In view point of immunohistochemistry, at 16 weeks, TNF-α positive cells were
observed in the connective tissue under the squamous cell carcinoma, and in the
adjacent portion of degeneration and necrosis of marginal area of the fibrosarcoma.
TNF-α positive cells in group 2 and 3 were much more distinct than in group 1.
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KEYWORD
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